AF5288

antibody from R&D Systems

Targeting: CSE1L CAS, CSE1, XPO2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5288

Provider

R&D Systems

Product name

Human/Mouse/Rat CSE1L/CAS Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human, mouse, and rat CSE1L in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P55060


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat CSE1L/CAS by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CSE1L/CAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5288) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CSE1L/CAS at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.