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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF5800 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Neurocan Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse and rat Neurocan in direct ELISAs and Western blots.
- Reactivity
- Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_031815- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies.
Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets.
BMP-Responsive Protease HtrA1 Is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response.
Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex.
Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates.
L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei.
Neurons and glia modify receptor protein-tyrosine phosphatase ζ (RPTPζ)/phosphacan with cell-specific O-mannosyl glycans in the developing brain.
Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Fontanil T, Mohamedi Y, Moncada-Pazos A, Cobo T, Vega JA, Cobo JL, García-Suárez O, Cobo J, Obaya ÁJ, Cal S
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;52(5):1003-1016
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;52(5):1003-1016
Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets.
Sonntag M, Blosa M, Schmidt S, Reimann K, Blum K, Eckrich T, Seeger G, Hecker D, Schick B, Arendt T, Engel J, Morawski M
BMC biology 2018 Sep 26;16(1):99
BMC biology 2018 Sep 26;16(1):99
BMP-Responsive Protease HtrA1 Is Differentially Expressed in Astrocytes and Regulates Astrocytic Development and Injury Response.
Chen J, Van Gulden S, McGuire TL, Fleming AC, Oka C, Kessler JA, Peng CY
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Apr 11;38(15):3840-3857
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Apr 11;38(15):3840-3857
Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex.
Ueno H, Suemitsu S, Okamoto M, Matsumoto Y, Ishihara T
Neuroscience 2017 Jul 4;355:161-174
Neuroscience 2017 Jul 4;355:161-174
Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates.
Bartels MF, Winterhalter PR, Yu J, Liu Y, Lommel M, Möhrlen F, Hu H, Feizi T, Westerlind U, Ruppert T, Strahl S
PloS one 2016;11(11):e0166119
PloS one 2016;11(11):e0166119
L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei.
Ebbers L, Satheesh SV, Janz K, Rüttiger L, Blosa M, Hofmann F, Morawski M, Griesemer D, Knipper M, Friauf E, Nothwang HG
The Journal of biological chemistry 2015 Sep 25;290(39):23692-710
The Journal of biological chemistry 2015 Sep 25;290(39):23692-710
Neurons and glia modify receptor protein-tyrosine phosphatase ζ (RPTPζ)/phosphacan with cell-specific O-mannosyl glycans in the developing brain.
Dwyer CA, Katoh T, Tiemeyer M, Matthews RT
The Journal of biological chemistry 2015 Apr 17;290(16):10256-73
The Journal of biological chemistry 2015 Apr 17;290(16):10256-73
Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Bekku Y, Oohashi T
Archives of histology and cytology 2010;73(2):95-102
Archives of histology and cytology 2010;73(2):95-102
Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Bekku Y, Oohashi T
Archives of histology and cytology 2010;73(2):95-102
Archives of histology and cytology 2010;73(2):95-102
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse/Rat Neurocan by Western Blot. Western blot shows lysates of rat embryonic hippocampal neurons. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurocan at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Neurocan in Mouse Brain. Neurocan was detected in perfusion fixed frozen sections of mouse brain (medulla) using Mouse/Rat Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5800) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
