AF4085

antibody from R&D Systems

Targeting: HAPLN4 BRAL2, KIAA1926

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4085

Provider

R&D Systems

Product name

Human HAPLN4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human HAPLN4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HAPLN1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q86UW8


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets.

Sonntag M, Blosa M, Schmidt S, Reimann K, Blum K, Eckrich T, Seeger G, Hecker D, Schick B, Arendt T, Engel J, Morawski M
BMC biology 2018 Sep 26;16(1):99

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

HAPLN4 in Human Brain. HAPLN4 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 3 µg/mL Goat Anti-Human HAPLN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4085) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.