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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AP8608a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP8608a, RRID:AB_10552941
- Product name
- RNF5 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.4 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of RNF5 Antibody (N-term) (Cat. #AP8608a) in K562 cell line lysates (35ug/lane). RNF5 (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of RNF5 (arrow) using rabbit polyclonal RNF5 Antibody (N-term) (Cat. #AP8608a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RNF5 gene.
- Primary Ab dilution
- 1:1000
