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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00008200-M06 - Provider product page
- Provider
- Abnova Corporation
- Product name
- GDF5 monoclonal antibody (M06), clone 1F4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant GDF5.
- Antigen sequence
APDLGQRPQGTRPGLAKAEAKERPPLARNVFRPGG
HSYGGGATNANARAKGGTGQTGGLTQPKKDEPKKL
PPRPGGPEPKPGHPPQTRQATARTVTPKGQ- Isotype
- IgG
- Antibody clone number
- 1F4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GDF5 monoclonal antibody (M06), clone 1F4. Western Blot analysis of GDF5 expression in HL-60.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GDF5 expression in transfected 293T cell line by GDF5 monoclonal antibody (M06), clone 1F4.Lane 1: GDF5 transfected lysate (Predicted MW: 55.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GDF5 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
