AF853

antibody from R&D Systems

Targeting: GDF5 BMP14, CDMP1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF853

Provider

R&D Systems

Product name

Mouse GDF-5/BMP-14 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse GDF-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF-8, rmGDF-9, recombinant human GDF-11, and rhGDF-15 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P43027


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Growth Differentiation Factor 5-Mediated Enhancement of Chondrocyte Phenotype Is Inhibited by Heparin: Implications for the Use of Heparin in the Clinic and in Tissue Engineering Applications.

Ayerst BI, Smith RA, Nurcombe V, Day AJ, Merry CL, Cool SM
Tissue engineering. Part A 2017 Apr;23(7-8):275-292

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Joint TGF-β type II receptor-expressing cells: ontogeny and characterization as joint progenitors.

Li T, Longobardi L, Myers TJ, Temple JD, Chandler RL, Ozkan H, Contaldo C, Spagnoli A
Stem cells and development 2013 May 1;22(9):1342-59

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Growth differentiation factor 5 regulates cardiac repair after myocardial infarction.

Zaidi SH, Huang Q, Momen A, Riazi A, Husain M
Journal of the American College of Cardiology 2010 Jan 12;55(2):135-43

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Freeze-dried tendon allografts as tissue-engineering scaffolds for Gdf5 gene delivery.

Basile P, Dadali T, Jacobson J, Hasslund S, Ulrich-Vinther M, Søballe K, Nishio Y, Drissi MH, Langstein HN, Mitten DJ, O'Keefe RJ, Schwarz EM, Awad HA
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Mar;16(3):466-73

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

GDF-5 in Mouse Embryo. GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.