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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG55780 - Provider product page
- Provider
- Arigo
- Product name
- anti-Proteasome 20S LMP2 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 193-219 (C-terminus) of Human Proteasome 20S LMP2
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) A431, and 2) Raji whole cell lysate stained with ARG55780 anti-Proteasome 20S LMP2 antibody at 1:1000 dilution. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HL-60 cell lysate stained with ARG55780 anti-Proteasome 20S LMP2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded Human prostate carcinoma stained with ARG55780 anti-Proteasome 20S LMP2 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HL60 cells stained with ARG55780 anti-Proteasome 20S LMP2 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
