Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-31515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- USP7 Monoclonal Antibody (GT481)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Antibody clone number
- GT481
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP7 in A) 293T whole cell extract, B) A431 whole cell extract, C) HeLa whole cell extract, D) HepG2 whole cell extract using USP7 monoclonal antibody (Product # MA5-31515) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of USP7 in A) 293T whole cell extract, B) A431 whole cell extract, C) HeLa whole cell extract, D) HepG2 whole cell extract using USP7 monoclonal antibody (Product # MA5-31515) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using USP7 Monoclonal Antibody (GT481) (Product # MA5-31515). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 Monoclonal Antibody (GT481) (Product # MA5-31515) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- USP7 Monoclonal Antibody (GT481) detects USP7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP7 protein stained by USP7 Monoclonal Antibody (GT481) (Product # MA5-31515) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of USP7 in HepG2 whole cell extracts with USP7 monoclonal antibody (Product # MA5-31515) using 5 µg of sample. Sample was then incubated with anti-mouse IgG secondary antibody.