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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-31986 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NIP30 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299.
- Concentration
- 0.42 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NIP30 Polyclonal Antibody (Product # PA5-31986). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: H1299. 12% SDS PAGE. NIP30 Polyclonal Antibody (Product # PA5-31986) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIP30 Polyclonal Antibody detects NIP30 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NIP30 protein stained by NIP30 Polyclonal Antibody (Product # PA5-31986) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NIP30 (Product # PA5-31986) antibody at 1:200 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
