LS-C289315

antibody from LSBio

Targeting: BRD2 BRD2-IT1, D6S113E, FSRG1, KIAA9001, NAT, RING3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Proximity ligation assay (Recommended by provider)

Antibody Data

Product number

LS-C289315

Provider

LSBio

Product name

Anti-BRD2 / RING3 Antibody (aa675-725) LS-C289315

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Bovine, Canine, Guinea Pig, Horse, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100µl

Storage

Store at 2-8°C for up to 1 year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human BRD2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD2 antibody used for IP at 6 ug/mg lysate. BRD2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-BRD2 antibody. For blotting immunoprecipitated BRD2, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.