Antibody data
- Product number
- PA5-102306
- Provider
- Invitrogen Antibodies
- Product name
- DOK4 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-102306
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DOK4 in MCF7 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with DOK4 polyclonal antibody (Product # PA5-102306).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DOK4 in MCF7 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with DOK4 polyclonal antibody (Product # PA5-102306).
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DOK4 in 293 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with DOK4 polyclonal antibody (Product # PA5-102306) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded DOK4 in human brain cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with DOK4 polyclonal antibody (Product # PA5-102306) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
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