MA3-812

antibody from Invitrogen Antibodies

Targeting: RXRB H-2RIIBP, NR2B2, RCoR-1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

MA3-812

Provider

Invitrogen Antibodies

Product name

Anti-RXRB Monoclonal Antibody (MOK13-17)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA3-813 detects cellular retinoic acid binding protein I (CRABPI) from human, mouse, monkey, feline, bovine, rabbit, rat, turtle and chicken tissues. This antibody does not react with chameleon, goldfish and frog. This antibody does not cross-react with cellular retinol binding protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein. MA3-813 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects a 16 kDa protein representing CRABPI from rat retinal supernatant. Immunohistochemical staining of CRABPI in rat retina with MA3-813 results in its localization to amacrine somata and laminae in the inner plexiform layer. The MA3-813 antigen is oxidized CRABPI from bovine retina.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

MOK13-17

Vial size

100 µg

Concentration

Lot Dependent

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Nuclear hormone retinoid X receptor (RXR) negatively regulates the glucose-stimulated insulin secretion of pancreatic ß-cells.

Miyazaki S, Taniguchi H, Moritoh Y, Tashiro F, Yamamoto T, Yamato E, Ikegami H, Ozato K, Miyazaki J
Diabetes 2010 Nov;59(11):2854-61

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G2/M arrest by 1,25-dihydroxyvitamin D3 in ovarian cancer cells mediated through the induction of GADD45 via an exonic enhancer.

Jiang F, Li P, Fornace AJ Jr, Nicosia SV, Bai W
The Journal of biological chemistry 2003 Nov 28;278(48):48030-40

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Ligand-dependent degradation of retinoid X receptors does not require transcriptional activity or coactivator interactions.

Osburn DL, Shao G, Seidel HM, Schulman IG
Molecular and cellular biology 2001 Aug;21(15):4909-18

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Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.

Thompson PD, Jurutka PW, Haussler CA, Whitfield GK, Haussler MR
The Journal of biological chemistry 1998 Apr 3;273(14):8483-91

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Dominant negative retinoid X receptor beta inhibits retinoic acid-responsive gene regulation in embryonal carcinoma cells.

Minucci S, Zand DJ, Dey A, Marks MS, Nagata T, Grippo JF, Ozato K
Molecular and cellular biology 1994 Jan;14(1):360-72

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Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene.

MacDonald PN, Dowd DR, Nakajima S, Galligan MA, Reeder MC, Haussler CA, Ozato K, Haussler MR
Molecular and cellular biology 1993 Sep;13(9):5907-17

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Inhibition of estrogen-responsive gene activation by the retinoid X receptor beta: evidence for multiple inhibitory pathways.

Segars JH, Marks MS, Hirschfeld S, Driggers PH, Martinez E, Grippo JF, Brown M, Wahli W, Ozato K
Molecular and cellular biology 1993 Apr;13(4):2258-68

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Heterodimerization of thyroid hormone (TH) receptor with H-2RIIBP (RXR beta) enhances DNA binding and TH-dependent transcriptional activation.

Hallenbeck PL, Marks MS, Lippoldt RE, Ozato K, Nikodem VM
Proceedings of the National Academy of Sciences of the United States of America 1992 Jun 15;89(12):5572-6

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A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR.

Mangelsdorf DJ, Umesono K, Kliewer SA, Borgmeyer U, Ong ES, Evans RM
Cell 1991 Aug 9;66(3):555-61