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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53130PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53130PU-N, RRID:AB_11147306
- Product name
- anti Otubain-1 (OTUB1) (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 65~95 amino acids from the N-terminal region of human Otubain-1 (OTUB1)
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of OTUB1 Antibody (N-term) Cat.-No AP53130PU-N in Y79 cell line lysates (35ug/lane). OTUB1 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with OTUB1 Antibody (N-term) Cat.-No AP53130PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using OTUB1 Antibody (N-term) Cat.-No AP53130PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
