Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00055611-M09 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055611-M09, RRID:AB_2236432
- Product name
- Mouse Monoclonal Otubain-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. OTUB1 - OTU domain, ubiquitin aldehyde binding 1 (1C12)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion.
Edelmann MJ, Kramer HB, Altun M, Kessler BM
The FEBS journal 2010 Jun;277(11):2515-30
The FEBS journal 2010 Jun;277(11):2515-30
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Otubain-1 Antibody (1C12) [H00055611-M09] - Analysis of OTUB1 expression in Hela S3 NE (Cat # L013V3).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Otubain-1 Antibody (1C12) [H00055611-M09] - Detection limit for recombinant GST tagged OTUB1 is 1 ng/ml as a capture antibody.