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LearnMA5-34651
antibody from Invitrogen Antibodies
Targeting: SEPTIN2
DIFF6, hNedd5, KIAA0158, NEDD5, Pnutl3, SEPT2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34651 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEPT2 Recombinant Rabbit Monoclonal Antibody (JB41-30)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Mouse brain, K562, SHSY-5Y, HUVEC, HepG2, human colon cancer tissue, human kidney tissue, rat brain tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB41-30
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Septin 2 in Positive control: Line 1: Mouse brain Line 2: SiHa Line 3: K562. Samples were incubated with Septin 2 monoclonal antibody (Product # MA5-34651), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Septin 2 in SHSY-5Y cells (red). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Septin 2 monoclonal antibody (Product # MA5-34651), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Septin 2 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Septin 2 monoclonal antibody (Product # MA5-34651), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Septin 2 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Septin 2 monoclonal antibody (Product # MA5-34651), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Septin 2 in paraffin-embedded rat brain tissue. Samples were incubated with Septin 2 monoclonal antibody (Product # MA5-34651), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Septin 2 in paraffin-embedded human kidney tissue. Samples were incubated with Septin 2 monoclonal antibody (Product # MA5-34651), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Septin 2 in paraffin-embedded human colon cancer tissue. Samples were incubated with Septin 2 monoclonal antibody (Product # MA5-34651), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Septin 2 in K562 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Septin 2 monoclonal antibody (Product # MA5-34651) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
