11397-1-AP
antibody from Invitrogen Antibodies
Targeting: SEPTIN2
DIFF6, hNedd5, KIAA0158, NEDD5, Pnutl3, SEPT2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 11397-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEPT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSKQQPTQF INPETPGYVG FANLPNQVHR KSVKKGFEFT LMVVGESGLG KSTLINSLFL TDLYPERVIS GAAEKIERTV QIEASTVEIE ERGVKLRLTV VDTPGYGDAI NCRDCFKTII SYIDEQFERY LHDESGLNRR HIIDNRVHCC FYFISPFGHG LKPLDVAFMK AIHNKVNIVP VIAKADTLTL KERERLKKRI LDEIEEHNIK IYHLPDAESD EDEDFKEQTR LLKASIPFSV VGSNQLIEAK GKKVRGRLYP WGVVEVENPE HNDFLKLRTM LITHMQDLQE VTQDLHYENF RSERLKRGGR KVENEDMNKD QILLEKEAEL RRMQEMIARM QAQMQMQMQG GDGDGGALGH HV (1-361 aa encoded by BC014455)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (SEPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11397-1-AP (SEPT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (SEPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (SEPT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C6 cells were subjected to SDS PAGE followed by western blot with 11397-1-AP (SEPT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 11397-1-AP (SEPT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result from Dr. Kevin Corbit. SEPT2 (11397-1-AP) marks the transition zone and cilium of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 11397-1-AP (SEPT2 antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 11397-1-AP (SEPT2 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 11397-1-AP (SEPT2 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 11397-1-AP (SEPT2 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug SEPT2 antibody (11397-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SEPT2 (IP:11397-1-AP, 3ug; Detection:11397-1-AP 1:1000) with mouse brain tissue lysate 5000ug.