NBP1-85212

antibody from Novus Biologicals

Targeting: SEPTIN2 DIFF6, hNedd5, KIAA0158, NEDD5, Pnutl3, SEPT2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85212

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85212, RRID:AB_11002313

Product name

Rabbit Polyclonal Septin-2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Septin-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Septin-2 is overexpressed in epithelial ovarian cancer and mediates proliferation via regulation of cellular metabolic proteins.

James NE, Cantillo E, Yano N, Chichester CO, DiSilvestro PA, Hovanesian V, Rao RSP, Kim KK, Moore RG, Ahsan N, Ribeiro JR
Oncotarget 2019 Apr 26;10(31):2959-2972

---

Septins arrange F-actin-containing fibers on the Chlamydia trachomatis inclusion and are required for normal release of the inclusion by extrusion.

Volceanov L, Herbst K, Biniossek M, Schilling O, Haller D, Nölke T, Subbarayal P, Rudel T, Zieger B, Häcker G
mBio 2014 Oct 7;5(5):e01802-14

---

Modulation of septin and molecular motor recruitment in the microtubule environment of the Taxol-resistant human breast cancer cell line MDA-MB-231.

Froidevaux-Klipfel L, Poirier F, Boursier C, Crépin R, Poüs C, Baudin B, Baillet A
Proteomics 2011 Oct;11(19):3877-86

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in human cell line HepG2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Septin-2 Antibody [NBP1-85212] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human small intestine shows weak cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Septin-2 Antibody [NBP1-85212] - Staining of human testis shows strong membranous/cytoplasmic positivity in cells in seminiferous ducts.