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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NB300-264 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-264, RRID:AB_2064244
- Product name
- Rabbit Polyclonal BIRC6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Programmed cell death in idiopathic dilated cardiomyopathy is mediated by suppression of the apoptosis inhibitor Apollon.
Aharinejad S, Andrukhova O, Lucas T, Zuckermann A, Wieselthaler G, Wolner E, Grimm M
The Annals of thoracic surgery 2008 Jul;86(1):109-14; discussion 114
The Annals of thoracic surgery 2008 Jul;86(1):109-14; discussion 114
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BIRC6 Antibody [NB300-264] - Samples: A) Whole cell lysate (WCL) and S100 extract (S100) from HeLa cells. B) Whole cell lysate (10 mg) from HeLa cells. Chemiluminescence with an exposure time of 10 minutes. B) Immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6 was confirmed by mass spectrometry.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BIRC6 Antibody [NB300-264] - Detection of Human and Mouse BIRC6 by Western Blot. Samples: Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody NB300-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BIRC6 Antibody [NB300-264] - Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody NB300-264 (lot 2) used for IP at 6 ug per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated BIRC6, NB300-264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Human ovarian tumor. Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Human breast carcinoma. Antibody used at a dilution of 1:5,000 (0.2ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BIRC6 Antibody [NB300-264] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BIRC6/Apollon used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB Coutnerstain: IHC Hematoxylin (blue).
