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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- TA500797 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500797, RRID:AB_11139901
- Product name
- Anti-LEMD3 (MAN1) mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)
- Antibody type
- Monoclonal
- Description
- Anti-LEMD3 (MAN1) mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LEMD3
- Isotype
- IgG
- Antibody clone number
- OTI4E1
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (RC212759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LEMD3 mouse monoclonal antibody (TA500797) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3(RC212759).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of LEMD3 by using TrueMab monoclonal anti-LEMD3 antibody (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-LEMD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500797, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-LEMD3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500797, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-LEMD3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500797, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-LEMD3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500797, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-LEMD3 antibody(TA500797),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
