Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-B3972 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ HMGIY / HMGA1 Antibody (aa9-21) LS-B3972
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Canine, Hamster, Horse, Porcine, Rabbit, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Staining (0.5 ug/ml) of Human Pancreas lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- HMGIY / HMGA1 antibody (0.5µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- HMGIY / HMGA1 antibody (0.5µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-HMGA1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-HMGA1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.