Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A302-305A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD23B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human RAD23B by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RAD23B (Cat. No. A302-305A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RAD23B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RAD23B antibody A302-305A used for IP at 10 µg/mg lysate. RAD23B was also immunoprecipitated by rabbit anti-RAD23B antibody A302-306A, which recognizes a downstream epitope. For blotting immunoprecipitated RAD23B, A302-306A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time 3 seconds.