GTX85831

antibody from GeneTex

Targeting: RAD23B HHR23B, HR23B, P58

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX85831

Provider

GeneTex

Proper citation

GeneTex Cat#GTX85831, RRID:AB_10722452

Product name

RAD23B antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Goat

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot of affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate. Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown). Approximately 33 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Human Prostate (formalin-fixed, paraffin-embedded) stained with RAD23B antibody (GTX85831) at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody LS-D3, alkaline phosphatase-streptavidin and chromogen.