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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-24188 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL23A1 Monoclonal Antibody (468642)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, 50% cross-reactivity with recombinant human Collagen XXV alpha 1 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 468642
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL23A1 in NCI‚H460 human large cell lung carcinoma cell line. Samples were incubated in COL23A1 monoclonal antibody (Product # MA5-24188) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. Specific bands were detected for Collagen XXIII α1 at approximately 75 and 60 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of COL23A1 in immersion fixed paraffin-embedded sections of human adenocarcinoma. Samples were incubated in COL23A1 monoclonal antibody (Product # MA5-24188) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.
