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- Immunohistochemistry [1]
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- Product number
- MA5-25662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFI35 Monoclonal Antibody (OTI1C9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1C9
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive Complete IFN-γR1 Deficiency.
Hahn K, Pollmann L, Nowak J, Nguyen AHH, Haake K, Neehus AL, Waqas SFH, Pessler F, Baumann U, Hetzel M, Casanova JL, Schulz A, Bustamante J, Ackermann M, Lachmann N
Molecular therapy. Methods & clinical development 2020 Jun 12;17:785-795
Molecular therapy. Methods & clinical development 2020 Jun 12;17:785-795
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFI35 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, MCF7 cells using 10 µg per lane. Samples were probed with IFI35 (Product # MA5-25662) monoclonal antibody at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFI35 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with IFI35 (Product # MA5-25662) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IFI35 in COS7 cells. Cells were transfected with a plasmid overexpressing IFI35 and probed with a IFI35 monoclonal antibody (Product # MA5-25662).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a IFI35 monoclonal antibody (Product # MA5-25662).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Evaluation of IFN-gammaR1 Expression and Functionality in Lv.SFFV.IFNgammaR1.iGFP Transduced IFN-gammaR1 -/- HeLa Cells (A) Representative histograms depicting IFN-gammaR1 (CD119) expression on WT (orange), IFN-gammaR1 -/- (knockout [KO], black), and Lv.SFFV.GFP (green) or Lv.SFFV.IFNgammaR1.iGFP (blue) transduced KO HeLa cells (unstained control cells are depicted in gray). (B) Quantification of median fluorescence intensities (MFIs) of IFN-gammaR1 (CD119) expression in WT, KO, and transduced HeLa cells (n = 3-4; mean +- SD, statistical analysis was performed using one-way-ANOVA with Tukey's post hoc testing. All shown values are compared to KO cells, *p
