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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-50670 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAT3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total GNAT3 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-GNAT3 Rabbit Polyclonal Antibody (Product # PA5-50670) and a 60kDa band corresponding to GNAT3 was observed in Mouse Tongue when compared to Mouse Colon, Mouse Testis, Rat Testis, Mouse Heart and Mouse Brain. Tissue extracts (30 µg lysate) of Mouse Colon (Lane 1), Mouse Testis (Lane 2), Rat Testis (Lane 3), Mouse Tongue (Lane 4), Mouse Heart (Lane 5) and Mouse Brain (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GNAT3 in paraffin embedded Human gastric cancer tissue using GNAT3 Polyclonal Antibody (Product # PA5-50670) at a 1:15 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GNAT3 in paraffin embedded Human thyroid cancer tissue using GNAT3 Polyclonal Antibody (Product # PA5-50670) at a 1:15 dilution.
