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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-47262 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GATA5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GATA-1, rhGATA-2, rhGATA-3, rhGATA-4, and rhGATA-6 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-humanGATA-5 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47262) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GATA-5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GATA5 in HEK293 human embryonic kidney cell line and HepG2 human hepatocellular carcinoma cell line. Samples were incubated in GATA5 polyclonal antibody (Product # PA5-47262) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for GATA‚5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GATA5 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in GATA5 polyclonal antibody (Product # PA5-47262) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assay of GATA5 in HeLa human cervical epithelial carcinoma cell line. Samples were immunoprecipitated with GATA5 polyclonal antibody (Product # PA5-47262) for 15 minutes in an ultrasonic bath using a dilution of 5 μg followed by a Biotinylated Anti-Goat IgG secondary antibody. Cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The mucin4 promoter was detected by standard PCR.
