H00001641-M01

antibody from Abnova Corporation

Targeting: DCX DBCN, DC, LISX, SCLH, XLIS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001641-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001641-M01, RRID:AB_463920

Product name

DCX monoclonal antibody (M01), clone 1G12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant DCX.

Antigen sequence

MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHC
SFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVY
AVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIY
TIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTK
NVNPNWSVNVKTSANMKAPQSLASSNSAQARENKD
FVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQV
LTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGD
DDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSA
TAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTS
SSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSL
DDSDSLGDSM


Isotype

IgG

Antibody clone number

1G12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

LC-MS/MS identification of doublecortin as abundant beta cell-selective protein discharged by damaged beta cells in vitro.

Jiang L, Brackeva B, Stangé G, Verhaeghen K, Costa O, Couillard-Després S, Rotheneichner P, Aigner L, Van Schravendijk C, Pipeleers D, Ling Z, Gorus F, Martens GA
Journal of proteomics 2013 Mar 27;80:268-80

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DCX expression in transfected 293T cell line by DCX monoclonal antibody (M01), clone 1G12.Lane 1: DCX transfected lysate(41.4 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to DCX on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol