Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F40640 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nephrin Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1191-1219 from the human protein was used as the immunogen for this Nephrin antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Nephrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Nephrin antibody western blot analysis in MDA-MB435 lysate. Predicted molecular weight ~150 kDa but often observed at a lower size.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Nephrin antibody western blot analysis in mouse kidney tissue lysate.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Nephrin antibody flow cytometric analysis of MDA-MB435 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
