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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- F53519 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nephrin Antibody
- Antibody type
- Monoclonal
- Antigen
- A portion of amino acids 1088-1117 from the human protein was used as the immunogen for this Nephrin antibody.
- Description
- Ascites
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 174CT2.1.1
- Vial size
- 100 µl
- Storage
- Aliquot the Nephrin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Nephrin antibody western blot analysis in SK-BR-3 lysate. Ab used at 1:100 dilution. Predicted molecular weight ~150 kDa but often observed at a lower size.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Nephrin antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
