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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ARG10579 - Provider product page
- Provider
- Arigo
- Product name
- anti-Nephrin antibody [Y17-R]
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide around the center of the fifth Ig-like domain of Human nephrin (between residues Cys465-Cys528)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- Y17-R
- Vial size
- 50 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 1) 50 ng, 2) 100 ng, and 3) 200 ng of recombinant Mouse nephrin (extracellular domain, Gln37 - Thr1049) stained with ARG10579 anti-Nephrin antibody [Y17-R].
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HEK293 cells stained with ARG10579 anti-Nephrin antibody [Y17-R] at 1:700 dilution.
