Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-77303 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77303, RRID:AB_11040198
- Product name
- Rabbit Polyclonal Nephrin Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Astragaloside IV inhibits glucose-induced epithelial-mesenchymal transition of podocytes through autophagy enhancement via the SIRT-NF-κB p65 axis.
Effect of extract of Pueraria tuberosa on expression of hypoxia inducible factor-1α and vascular endothelial growth factor in kidney of diabetic rats.
Vanillic Acid Ameliorates Cationic Bovine Serum Albumin Induced Immune Complex Glomerulonephritis in BALB/c Mice.
Wang X, Gao Y, Tian N, Wang T, Shi Y, Xu J, Wu B
Scientific reports 2019 Jan 23;9(1):323
Scientific reports 2019 Jan 23;9(1):323
Effect of extract of Pueraria tuberosa on expression of hypoxia inducible factor-1α and vascular endothelial growth factor in kidney of diabetic rats.
Shukla R, Pandey N, Banerjee S, Tripathi YB
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Sep;93:276-285
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2017 Sep;93:276-285
Vanillic Acid Ameliorates Cationic Bovine Serum Albumin Induced Immune Complex Glomerulonephritis in BALB/c Mice.
Motiram Kakalij R, Tejaswini G, Patil MA, Dinesh Kumar B, Diwan PV
Drug development research 2016 Jun;77(4):171-9
Drug development research 2016 Jun;77(4):171-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue lysate with Nephrin antibody at 1 u/mL in the (A) absence and (B) presence of blocking peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nephrin Antibody [NBP1-77303] - Loading: 15 ug of lysates per lane. Antibodies: Nephrin (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nephrin Antibody [NBP1-77303] - Nephrin expression detected by anti-nephrin antibodies in type 1 diabetic rats. Nephrin was down-regulated in the vehicle-treated diabetic rats as compared to the control nondiabetic rats. However, this decrease in nephrin protein expression was markedly increased by curcumin treatment (P
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nephrin Antibody [NBP1-77303] - The expression of nephrin detected by anti-nephrin antibodies did not change in ZO rats as compared to the control rats.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nephrin Antibody [NBP1-77303] - Analysis was used to access the protein expression level of nephrin with anti-nephrin antibodies. Nephrin expression was down-regulated by PEGF treatment (NGP or HGP), which was reversed by the addition of C3 transferase.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nephrin Antibody [NBP1-77303] - Mouse kidney tissue with Nephrin antibody at 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nephrin Antibody [NBP1-77303] - Staining of rat kidney tissue with antibody at 5 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nephrin Antibody [NBP1-77303] - Protein analysis for nephrin by immunohistochemistry with anti-nephrin antibodies in the kidney of wild-type or AMPD2-deficient mice at 2, 12 or 24 weeks of age. No difference between wild-type and AMPD2-deficient mice at any age was observed.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nephrin Antibody [NBP1-77303] - Glomerular cells of Atgl (-/-) mice were double labeled with TUNEL staining (dark brown nucleus indicated by red arrows) and immunofluorescence staining of nephrin detected by anti-nephrin antibodies (pink cytoplasm indicated by green arrows) as a marker for podocytes. Colocalization of TUNEL-positive cells and nephrin proved that apoptosis was induced in Atgl(-/-) mice as compared to WT mice.