Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Gel shift [1]
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Validation data
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- Product number
- LS-C348746 - Provider product page
- Provider
- LSBio
- Product name
- Anti-E2F7 Antibody LS-C348746
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100µl
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- The antibody immunoprecipitates endogenous E2F7-A. 500 ug of whole cell extracts from K562 cells were immunoprecipitated with 2 ul of the crude rabbit serum or the corresponding pre-immune serum (PI). Immunoprecipitated proteins were fractionated on 8% SDSPAGE and transferred to nitrocellulose membranes. The membranes were then probed with a previously described anti-E2F7 antibody (1). Peroxidase-conjugated anti-rabbit antibodies followed by ECL were then used. The arrow points to immunoprecipitated E2F7-A.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- The anti-E2F7-A antibody is able to supershift a DNA-protein complex that contains E2F7-A. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from mock transfected Saos-2 cells (-) or nuclear extracts from Saos-2 cells transfected with pcDNA3-HAE2F7- A (+) were analyzed by EMSA. Extracts were preincubated in the absence (None) or in the presence of an antibody to the HA tag or the anti-E2F7-A antibody. The arrow indicates the position of the HA-E2F7-A containing complex, whereas arrowheads indicate the position of supershifted complexes.