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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP21994PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21994PU-N, RRID:AB_10757604
- Product name
- anti ARID5B / MRF2 (500-550)
- Antibody type
- Polyclonal
- Antigen
- Amino acids 500-550 of MRF2
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of MRF2 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using AP21994PU-N at 2 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of MRF2 in human U87 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate using AP21994PU-N at 3 µg/ml, 3 µg/ml and 2 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with MRF2 antibody (Cat.-No.: AP21994PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
