BS8367

antibody from Bioworld Technology, Inc
Targeting: PPP1R12A M130, MBS, MYPT1
Provider product page for BS8367
Western blot
Immunohistochemistry

Antibody data

Product number
BS8367 - Provider product page
Provider
Bioworld Technology, Inc
Product name
PPP1R12A polyclonal antibody
Antibody type
Polyclonal
Antigen
Recombinant full length Human PPP1R12A.
Description
Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. MYPT1 promotor contains one Sp1 transcription factor binding site, suggesting that MYPT1 is a housekeeping gene. Myotonic dystrophy protein kinase phosphorylates MYPT1 at Tyrosine 654 to regulate Myosin II phosphorylation. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphoryl-ates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated Myosin light chain to MYPT1. PP1c interacts with at least four binding sties on the amino-terminus of MYPT1. MYPT2, a novel isoform of MYPT1, also interacts with PPlc. MYPT1 is localized on stress fibers; it is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Isotype
IgG
Vial size
100ul
Concentration
1 mg/ml
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
PPP1R12A protein structure - BS8367 shown in red.