LS-C368437

antibody from LSBio

Targeting: NFAT5 KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP, TONEBP

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C368437

Provider

LSBio

Product name

NFAT5 Antibody (C-Terminus) LS-C368437

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of NFAT5 expression in HT29 (A); Jurkat (B); HeLa (C) whole cell lysates.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of NFAT5 expression in HT29 (A); Jurkat (B); HeLa (C) whole cell lysates.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of NFAT5 expression in HT29 (A); Jurkat (B); HeLa (C) whole cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of NFAT5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of NFAT5 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.