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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-35920 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 0.8 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAP4 Polyclonal Antibody detects MAP4 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.5 % SDS-PAGE. MAP4 Polyclonal Antibody (Product # PA5-35920) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAP4 Polyclonal Antibody detects MAP4 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.5 % SDS-PAGE. MAP4 Polyclonal Antibody (Product # PA5-35920) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAP4 Polyclonal Antibody detects MAP4 protein at cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: MAP4 protein stained by MAP4 Polyclonal Antibody (Product # PA5-35920) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAP4 Polyclonal Antibody detects MAP4 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. MAP4 Polyclonal Antibody (Product # PA5-35920) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
