Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-89483 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89483, RRID:AB_11032702
- Product name
- Rabbit Polyclonal MAP4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MAP4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP4 Antibody [NBP1-89483] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human heart muscle shows cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human kidney, skeletal muscle, soft tissues and testis using Anti-MAP4 antibody NBP1-89483 (A) shows similar protein distribution across tissues to independent antibody NBP1-85874 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human skeletal muscle.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human soft tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4 Antibody [NBP1-89483] - Staining of human kidney.