Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14577-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14577-1-AP, RRID:AB_2270189
- Product name
- SF3B3 antibody
- Antibody type
- Polyclonal
- Description
- SF3B3 antibody (Cat. #14577-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.
Enterotoxigenic Bacteroidesfragilis Promotes Intestinal Inflammation and Malignancy by Inhibiting Exosome-Packaged miR-149-3p.
Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.
RNA structure replaces the need for U2AF2 in splicing.
SF3B1 association with chromatin determines splicing outcomes.
Rearrangements within human spliceosomes captured after exon ligation.
Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.
Zhang Q, Zhang J, Ye J, Li X, Liu H, Ma X, Wang C, He K, Zhang W, Yuan J, Zhao Y, Xu H, Liu Q
Molecular neurodegeneration 2021 Sep 22;16(1):66
Molecular neurodegeneration 2021 Sep 22;16(1):66
Enterotoxigenic Bacteroidesfragilis Promotes Intestinal Inflammation and Malignancy by Inhibiting Exosome-Packaged miR-149-3p.
Cao Y, Wang Z, Yan Y, Ji L, He J, Xuan B, Shen C, Ma Y, Jiang S, Ma D, Tong T, Zhang X, Gao Z, Zhu X, Fang JY, Chen H, Hong J
Gastroenterology 2021 Nov;161(5):1552-1566.e12
Gastroenterology 2021 Nov;161(5):1552-1566.e12
Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells.
Yu NK, McClatchy DB, Diedrich JK, Romero S, Choi JH, MartÃnez-Bartolomé S, Delahunty CM, Muotri AR, Yates JR 3rd
iScience 2021 Nov 19;24(11):103321
iScience 2021 Nov 19;24(11):103321
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
Zheng YZ, Xue MZ, Shen HJ, Li XG, Ma D, Gong Y, Liu YR, Qiao F, Xie HY, Lian B, Sun WL, Zhao HY, Yao L, Zuo WJ, Li DQ, Wang P, Hu X, Shao ZM
Cancer research 2018 Jun 15;78(12):3190-3206
Cancer research 2018 Jun 15;78(12):3190-3206
Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.
Chen R, Liu Y, Zhuang H, Yang B, Hei K, Xiao M, Hou C, Gao H, Zhang X, Jia C, Li L, Li Y, Zhang N
Nucleic acids research 2017 Sep 29;45(17):9947-9959
Nucleic acids research 2017 Sep 29;45(17):9947-9959
RNA structure replaces the need for U2AF2 in splicing.
Lin CL, Taggart AJ, Lim KH, Cygan KJ, Ferraris L, Creton R, Huang YT, Fairbrother WG
Genome research 2016 Jan;26(1):12-23
Genome research 2016 Jan;26(1):12-23
SF3B1 association with chromatin determines splicing outcomes.
Kfir N, Lev-Maor G, Glaich O, Alajem A, Datta A, Sze SK, Meshorer E, Ast G
Cell reports 2015 Apr 28;11(4):618-29
Cell reports 2015 Apr 28;11(4):618-29
Rearrangements within human spliceosomes captured after exon ligation.
Ilagan JO, Chalkley RJ, Burlingame AL, Jurica MS
RNA (New York, N.Y.) 2013 Mar;19(3):400-12
RNA (New York, N.Y.) 2013 Mar;19(3):400-12
Breaking up the C complex spliceosome shows stable association of proteins with the lariat intron intermediate.
Coltri P, Effenberger K, Chalkley RJ, Burlingame AL, Jurica MS
PloS one 2011 Apr 19;6(4):e19061
PloS one 2011 Apr 19;6(4):e19061
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14577-1-AP(SF3B3 antibody) at dilution of 1:10000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 14577-1-AP(SF3B3 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 14577-1-AP(SF3B3 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue