Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34752 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SF3B3 Recombinant Rabbit Monoclonal Antibody (JG63-53)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Jurkat, Hela, A549, rat brain tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse testis tissue, LOVO.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG63-53
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SF3B3 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with SF3B3 monoclonal antibody (Product # MA5-34752), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SF3B3 in paraffin-embedded rat brain tissue. Samples were incubated with SF3B3 monoclonal antibody (Product # MA5-34752), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SF3B3 in paraffin-embedded mouse testis tissue. Samples were incubated with SF3B3 monoclonal antibody (Product # MA5-34752), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SF3B3 in paraffin-embedded human tonsil tissue. Samples were incubated with SF3B3 monoclonal antibody (Product # MA5-34752), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SF3B3 in paraffin-embedded human kidney tissue. Samples were incubated with SF3B3 monoclonal antibody (Product # MA5-34752), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SF3B3 in paraffin-embedded human colon cancer tissue. Samples were incubated with SF3B3 monoclonal antibody (Product # MA5-34752), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of SF3B3 in LOVO cells (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple). Samples were incubated with SF3B3 monoclonal antibody (Product # MA5-34752) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
