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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB22491P-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Human NKp44/NCR2 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human NKp44/NCR2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp46, rhNKp80, or recombinant mouse NKp46 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
CAB52289- Isotype
- IgG
- Antibody clone number
- 253415
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-β-dependent emperipolesis in HBV cirrhotic patients.
Altered expression of miR-181a and miR-146a does not change the expression of surface NCRs in human NK cells.
Shi J, Zhao J, Zhang X, Cheng Y, Hu J, Li Y, Zhao X, Shang Q, Sun Y, Tu B, Shi L, Gao B, Wang FS, Zhang Z
Scientific reports 2017 Mar 14;7:44544
Scientific reports 2017 Mar 14;7:44544
Altered expression of miR-181a and miR-146a does not change the expression of surface NCRs in human NK cells.
Rady M, Watzl C, Claus M, Khorshid O, Mahran L, Abou-Aisha K
Scientific reports 2017 Feb 1;7:41381
Scientific reports 2017 Feb 1;7:41381
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of NKp44/NCR2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with Recombinant Human IL-2 (Catalog # 202-IL) were stained with Mouse Anti-Human NKp44/NCR2 PE-conjugated Monoclonal Antibody (Catalog # FAB22491P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
