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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-77939 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRINP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: MCF-7
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DBC1 in MCF-7 whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with DBC1 polyclonal antibody (Product # PA5-77939) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DBC1 antibody detects DBC1 protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract.7.5 % SDS-PAGE. DBC1 antibody BRINP1 Polyclonal Antibody (Product # PA5-77939) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRINP1 Polyclonal Antibody detects DBC1 protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 100% MeOH for 5 min. Green: DBC1 protein stained by BRINP1 Polyclonal Antibody (Product # PA5-77939) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRINP1 Polyclonal Antibody detects DBC1 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BRINP1 Polyclonal Antibody (Product # PA5-77939) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRINP1 Polyclonal Antibody detects DBC1 protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. BRINP1 Polyclonal Antibody (Product # PA5-77939) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
