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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF1132 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Netrin-4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Netrin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximatley 25% cross-reactivity with recombinant human Netrin-4 and less than 1% cross-reactivity with recombinant mouse Netrin-1, recombinant chicken Netrin-2, and recombinant mouse Netrin-G1a.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Dorsal horn interneuron-derived Netrin-4 contributes to spinal sensitization in chronic pain via Unc5B.
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
Hayano Y, Takasu K, Koyama Y, Yamada M, Ogawa K, Minami K, Asaki T, Kitada K, Kuwabara S, Yamashita T
The Journal of experimental medicine 2016 Dec 12;213(13):2949-2966
The Journal of experimental medicine 2016 Dec 12;213(13):2949-2966
A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
Hägerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams RH, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
The EMBO journal 2013 Mar 6;32(5):629-44
The EMBO journal 2013 Mar 6;32(5):629-44
Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia.
Hoang S, Liauw J, Choi M, Choi M, Guzman RG, Steinberg GK
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2009 Feb;29(2):385-97
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2009 Feb;29(2):385-97
Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
Wang W, Reeves WB, Ramesh G
American journal of physiology. Renal physiology 2008 Apr;294(4):F739-47
American journal of physiology. Renal physiology 2008 Apr;294(4):F739-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Netrin-4 in Mouse Brain. Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 15 µg/mL Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
