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LearnH00023304-M01-100ug
antibody from Novus Biologicals
Targeting: UBR2
bA49A4.1, C6orf133, dJ392M17.3, KIAA0349
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00023304-M01-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal UBR2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. UBR2 - ubiquitin protein ligase E3 component n-recognin 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 over-expressed 293 cell line, cotransfected with UBR2 Validated Chimera RNAi ( Cat # H00023304-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UBR2 monoclonal antibody (M01), clone 4G4 (Cat # H00023304-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of UBR2 expression in transfected 293T cell line by UBR2 monoclonal antibody (M01), clone 4G4.Lane 1: UBR2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: UBR2 Antibody (4G4) [H00023304-M01] - Detection limit for recombinant GST tagged UBR2 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: UBR2 Antibody (4G4) [H00023304-M01] - Analysis of monoclonal antibody to UBR2 on HeLa cell . Antibody concentration 10 ug/ml.
