703650

antibody from Invitrogen Antibodies

Targeting: NRXN1 Hs.22998, KIAA0578

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703650

Provider

Invitrogen Antibodies

Product name

NRXN1 Recombinant Rabbit Monoclonal Antibody (22H29L23)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

22H29L23

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of NRXN1 was achieved by transfecting U-87 MG cells with specific siRNA (Silencer® select Product # s17948, s17947). Immunofluorescence analysis was performed on U-87VMG cells (untransfected, panel a-d), transfected with NRXN1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labelled with Anti-NRXN1 Recombinant Rabbit Monoclonal Antibody (Product # 703650, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of signal was observed upon siRNA mediated knockdown (panel i-l) confirming specificity of the antibody to NRXN1 (green). The images were captured at 60X magnification.