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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-111199 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ESDSPLAPGP FSKLLQPDMS HHYESWFRPT HPGAEDGSWW DLHPGTSWMD LPHTQGALTS PGHPGALQAG LGGYVGDHQL CAP
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SP6 in control (vector only transfected HEK293T lysate) and SP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404600) using SP6 Polyclonal Antibody (Product # PA5-111199).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SP6 in control (vector only transfected HEK293T lysate) and SP6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404600) using SP6 Polyclonal Antibody (Product # PA5-111199).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP6 in human cell line CACO-2 using SP6 Polyclonal Antibody (Product # PA5-111199) shows localization to nucleoplasm, centrosome and mitotic spindle.
