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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC1420P - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha-Smooth Muscle Actin PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha-Smooth Muscle Actin.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A4
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation.
A novel flow cytometry tool for fibrosis scoring through hepatic stellate cell differentiation.
Attenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung disease.
Reduced suppressive effect of β(2)-adrenoceptor agonist on fibrocyte function in severe asthma.
MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
Natural killer cell-dependent anti-fibrotic pathway in liver injury via Toll-like receptor-9.
Loss of endogenous bone morphogenetic protein-6 aggravates renal fibrosis.
Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
Circulating angiogenic precursors in idiopathic pulmonary arterial hypertension.
Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
Yuan Y, Li M, To CH, Lam TC, Wang P, Yu Y, Chen Q, Hu X, Ke B
Investigative ophthalmology & visual science 2018 Jul 2;59(8):3619-3629
Investigative ophthalmology & visual science 2018 Jul 2;59(8):3619-3629
A novel flow cytometry tool for fibrosis scoring through hepatic stellate cell differentiation.
Amer J, Salhab A, Doron S, Morali G, Safadi R
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2018 Apr;93(4):427-435
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2018 Apr;93(4):427-435
Attenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung disease.
Oak P, Pritzke T, Thiel I, Koschlig M, Mous DS, Windhorst A, Jain N, Eickelberg O, Foerster K, Schulze A, Goepel W, Reicherzer T, Ehrhardt H, Rottier RJ, Ahnert P, Gortner L, Desai TJ, Hilgendorff A
EMBO molecular medicine 2017 Nov;9(11):1504-1520
EMBO molecular medicine 2017 Nov;9(11):1504-1520
Reduced suppressive effect of β(2)-adrenoceptor agonist on fibrocyte function in severe asthma.
Lo CY, Michaeloudes C, Bhavsar PK, Huang CD, Chang PJ, Wang CH, Kuo HP, Chung KF
Respiratory research 2017 Nov 21;18(1):194
Respiratory research 2017 Nov 21;18(1):194
MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.
Kwan P, Ding J, Tredget EE
PloS one 2015;10(4):e0123054
PloS one 2015;10(4):e0123054
Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
Huang L, Nakayama H, Klagsbrun M, Mulliken JB, Bischoff J
Stem cells (Dayton, Ohio) 2015 Jan;33(1):133-45
Stem cells (Dayton, Ohio) 2015 Jan;33(1):133-45
Natural killer cell-dependent anti-fibrotic pathway in liver injury via Toll-like receptor-9.
Abu-Tair L, Axelrod JH, Doron S, Ovadya Y, Krizhanovsky V, Galun E, Amer J, Safadi R
PloS one 2013;8(12):e82571
PloS one 2013;8(12):e82571
Loss of endogenous bone morphogenetic protein-6 aggravates renal fibrosis.
Dendooven A, van Oostrom O, van der Giezen DM, Leeuwis JW, Snijckers C, Joles JA, Robertson EJ, Verhaar MC, Nguyen TQ, Goldschmeding R
The American journal of pathology 2011 Mar;178(3):1069-79
The American journal of pathology 2011 Mar;178(3):1069-79
Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
Semenov OV, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch AH, Malek A
American journal of obstetrics and gynecology 2010 Feb;202(2):193.e1-193.e13
American journal of obstetrics and gynecology 2010 Feb;202(2):193.e1-193.e13
Circulating angiogenic precursors in idiopathic pulmonary arterial hypertension.
Asosingh K, Aldred MA, Vasanji A, Drazba J, Sharp J, Farver C, Comhair SA, Xu W, Licina L, Huang L, Anand-Apte B, Yoder MC, Tuder RM, Erzurum SC
The American journal of pathology 2008 Mar;172(3):615-27
The American journal of pathology 2008 Mar;172(3):615-27
Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ Jr, Yokota SJ, Bankert RB
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5552-62
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5552-62
Characterization of human lung tumor-associated fibroblasts and their ability to modulate the activation of tumor-associated T cells.
Nazareth MR, Broderick L, Simpson-Abelson MR, Kelleher RJ Jr, Yokota SJ, Bankert RB
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5552-62
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5552-62
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of alpha-Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human alpha-Smooth Muscle Actin PE-conjugated Monoclonal Antibody (Catalog # IC1420P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
