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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-22236 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PSME4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Novel proteasome assembly chaperone mutations in PSMG2/PAC2 cause the autoinflammatory interferonopathy CANDLE/PRAAS4.
Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes.
de Jesus AA, Brehm A, VanTries R, Pillet P, Parentelli AS, Montealegre Sanchez GA, Deng Z, Paut IK, Goldbach-Mansky R, Krüger E
The Journal of allergy and clinical immunology 2019 May;143(5):1939-1943.e8
The Journal of allergy and clinical immunology 2019 May;143(5):1939-1943.e8
Inhibition of Proteasome Activity Induces Formation of Alternative Proteasome Complexes.
Welk V, Coux O, Kleene V, Abeza C, Trümbach D, Eickelberg O, Meiners S
The Journal of biological chemistry 2016 Jun 17;291(25):13147-59
The Journal of biological chemistry 2016 Jun 17;291(25):13147-59
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PSME4 Antibody [NBP2-22236] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22236 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PSME4 Antibody [NBP2-22236] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. NBP2-22236 used for IP at 6 ug/mg lysate. PSME4 was also immunoprecipitated by rabbit anti-PSME4 antibody. For blotting immunoprecipitated PSME4, NBP2-22236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-22236] - Human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- PSME4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
