Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-89458 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSME4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LYPHQVPLVL QVLKQTARSS SWHARYTVLT YLQTMVFYNL FIFLNNEDAV KDIRWLVISL LEDEQLEVRE MAATTLSGLL QCNFLTMDSP MQIHFEQLCK TKLPKKRKRD PGSVGDTIPS AELVKRHAGV LGLGACVLSS PYDVPTWMPQ LLMNLSAHLN DPQPIEMTVK KTLSNFRRTH HDNWQEHKQQ FTDDQLLVLT DLLVSPCYYA; Positive Samples: MCF-7, U-87MG, 293T, HeLa, Mouse thymus, Mouse brain, Mouse heart, Mouse kidney, Rat liver; Cellular Location: Cytoplasm, Nucleus, Nucleus speckle, cytosol
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.38 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of extracts of various cell lines, using PSME4 Polyclonal antibody (Product # PA5-89458) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.