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LearnPA5-31190
antibody from Invitrogen Antibodies
Targeting: MARCHF5
FLJ20445, MARCH-V, MARCH5, MITOL, RNF153
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-31190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MARCH5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HeLa MF. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MARCH5 using 20 µg of A) HeLa and B) HeLa membrane lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a MARCH5 polyclonal antibody (Product # PA5-31190) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MARCH5 in methanol-fixed HeLa cells using a MARCH5 polyclonal antibody (Product # PA5-31190) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
