PAB1954

antibody from Abnova Corporation

Targeting: ZIC3 HTX, HTX1, ZNF203

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB1954

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB1954, RRID:AB_1582034

Product name

ZIC3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ZIC3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Characterization of the interactions of human ZIC3 mutants with GLI3.

Zhu L, Zhou G, Poole S, Belmont JW
Human mutation 2008 Jan;29(1):99-105

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Identification of a novel role of ZIC3 in regulating cardiac development.

Zhu L, Harutyunyan KG, Peng JL, Wang J, Schwartz RJ, Belmont JW
Human molecular genetics 2007 Jul 15;16(14):1649-60

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Nuclear import and export signals are essential for proper cellular trafficking and function of ZIC3.

Bedard JE, Purnell JD, Ware SM
Human molecular genetics 2007 Jan 15;16(2):187-98

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of ZIC3 polyclonal antibody (Cat # PAB1954) in HL-60 cell line lysates (35 ug/lane).ZIC3 (arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ZIC3 polyclonal antibody (Cat # PAB1954), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)